Method Development and Validation for the Simultaneous Determination of Omeprazole and Domperidone in Solid Dosage Form by Rp-hplc

The present work describes a simple Reverse Phase HPLC method for the determination of Omeprazole and Domperidone from capsule formulations. The determination was carried out on a Gasco, ODS, C-18 (250× 4.5 mm, 5 micron) column using a mobile phase of Acetonitrile: 0.05M ammonium acetate buffer (pH4) in the ratio of (85:15). The flow rate and runtime were 1 ml/min and 10 min, respectively. The eluent was monitored at 280 nm. The method was reproducible, with good resolution between Omeprazole and Domperidone. The detector response was found to be linear in the concentration range of 4-12 μg/ml for Omeprazole and 8-16 μg/ml for Domperidone. Keyword: Domperidone (DOM), Omeprazole (OME), Acetonitrile, Jasco HPLC (2080 Plus) and 0.05 ammonium acetate buffer (pH 4)